High-Resolution Melting (HRM) Curve Assay for the Identification of Eight Fusarium Species Causing Ear Rot in Maize
نویسندگان
چکیده
منابع مشابه
The Major Fusarium Species Causing Maize Ear and Kernel Rot and Their Toxigenicity in Chongqing, China
Fusarium verticillioides, F. proliferatum, and F. meridionale were identified as the predominant fungi among 116 Fusarium isolates causing maize ear and kernel rot, a destructive disease in Chongqing areas, China. The toxigenic capability and genotype were determined by molecular amplification and toxin assay. The results showed that the key toxigenic gene FUM1 was detected in 47 F. verticillio...
متن کاملIdentification of Pathogenic Fusarium spp. Causing Maize Ear Rot and Potential Mycotoxin Production in China
Ear rot is a serious disease that affects maize yield and grain quality worldwide. The mycotoxins are often hazardous to humans and livestock. In samples collected in China between 2009 and 2014, Fusarium verticillioides and F. graminearum species complex were the dominant fungi causing ear rot. According to the TEF-1α gene sequence, F. graminearum species complex in China included three indepe...
متن کاملMolecular Basis of Resistance to Fusarium Ear Rot in Maize
The impact of climate change has been identified as an emerging issue for food security and safety, and the increased incidence of mycotoxin contamination in maize over the last two decades is considered a potential emerging hazard. Disease control by chemical and agronomic approaches is often ineffective and increases the cost of production; for this reason the exploitation of genetic resistan...
متن کاملHigh-Resolution Melting Curve Analysis for Identification of Pasteurellaceae Species in Experimental Animal Facilities
Pasteurellaceae are among the most prevalent bacterial pathogens isolated from mice housed in experimental animal facilities. Reliable detection and differentiation of Pasteurellaceae are essential for high-quality health monitoring. In this study, we combined a real-time PCR assay amplifying a variable region in the 16S rRNA sequence with high-resolution melting curve analysis (HRM) to identif...
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ژورنال
عنوان ژورنال: Pathogens
سال: 2020
ISSN: 2076-0817
DOI: 10.3390/pathogens9040270